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Biomiga 2x qPCR Mix SBGR, 100 Rxn, Kit Includes Antibody Inactivated Hot Start Compatible with BioRad CFX Connect, CFX96 Touch, Rotor Gene Q, Illumina Eco qPCR, Eppendorf Mastercycler, Roche Lightcycler.
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qPCR Master Mix: Is a ready-to-use reaction cocktail that contains all components except primersand template for real-time quantitative PCR. The proprietary buffer and stabilizers have been optimizedfor SYBR Green qPCR to deliver maximum PCR efficiency, sensitivity and robust fluorescent signal. The proprietary 2X qPCR Master Mix contains Hot Start Taq DNA polymerase, SYBR Green 1 Dye,dNTPs, Mg2+, enhancer, and stabilizer. The Hot Start Taq DNA polymerase is an antibody-inactivatedhot-start enzyme designed to block polymerase activity at ambient temperature. Once the PCR step reaches denaturation temperature, Taq DNA polymerase activity is restored and the resulting PCRexhibits higher sensitivity, specificity and yield. The system enables highly sensitive detection fromfew copies of DNA template, with a broad dynamic range that supports accurate quantification oftarget sequences. Sufficient reagents are provided for 50 amplification reactions of 50 μl each.
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