Details

Bacterial gDNA Isolation Kits: The EZgene^TM Bacterial gDNA kit provides a rapid and reliable method
for isolating high-quality total cellular DNA from a wide variety of bacterial species. Bacterial cells are
grown to log-phase and harvested. The bacterial cell wall is digested by lysozyme and Proteinase K.
Following lysis, binding conditions are adjusted and the sample is applied to a DNA column. Key to
this kit is our proprietary DNA binding system that allows the high efficient binding of DNA to our ezBind^TM
 matrix while proteins and other contaminates are removed under certain optimal conditions. Nucleic acids
 are easily eluted with sterile water or Elution Buffer. Purified DNA is suitable for PCR, restriction digestion,
and hybridization techniques. Each DNA column can bind approximately 100 μg genomic DNA. Up to 1 x 10⁹
 bacterial cells can be processed per column. The system combines the reversible nucleic acid-binding properties
of our ezBind matrix with the speed and versatility of spin column technology to yield up to approximately
15-30 μg of DNA with an OD_260/280 ratio of 1.7-1.9. The kit will isolate all cellular DNA, including plasmid DNA.