Details

Endotoxins, are cell membrane components of Gram-negative bactgeria such as E.coli. Endotoxins are released during the lysis step of plasmid purification and reduce transfection efficiencies in endotoxin-sensitive cell lines. Endotoxions represent a non-controllable variable in transfection experiment set-up making results difficult to compare and interpret.

IBI Fast-Ion Endo-Free Plasmid kits are designed to deliver clear cell lysate with minimal genomic DNA or RNA contaminants. The pre-packed anion exchange resin columns will purify DNA from 50-100ml of bacterial cultures.