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Product Code : IB47112 Price: $ 96.73


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Fast-Ion Plasmid Kits (Endotoxin Free, Kit Contains: 2 MIDI PLasmid Columns, 10ml PM1 Buffer, 10ml PM2 Buffer, 10ml PM3 Buffer, 4ml PER Buffer, 12ml PEQ Buffer, 30ml PW Buffer, 25ml PEL Buffer, RNase A Added to the PM1 Buffer (store at 4C).

Details

    Endotoxins, are cell membrane components of Gram-negative bactgeria such as E.coli. Endotoxins are released during the lysis step of plasmid purification and reduce transfection efficiencies in endotoxin-sensitive cell lines. Endotoxions represent a non-controllable variable in transfection experiment set-up making results difficult to compare and interpret.

    IBI Fast-Ion Endo-Free Plasmid kits are designed to deliver clear cell lysate with minimal genomic DNA or RNA contaminants. The pre-packed anion exchange resin columns will purify DNA from 50-100ml of bacterial cultures.

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